Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all KIF14 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 900 and 950 of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIF14 Antibody - BSA Free

Western Blot: KIF14 Antibody [NB100-254]

Western Blot: KIF14 Antibody [NB100-254]

Western Blot: KIF14 Antibody [NB100-254] - Detection of Human KIF14 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIF14 antibody NB100-254 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: KIF14 Antibody [NB100-254]

Immunoprecipitation: KIF14 Antibody [NB100-254]

Immunoprecipitation: KIF14 Antibody [NB100-254] - Detection of human KIF14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIF14 antibody NB100-254 (lot 2) used for IP at 6 ug per reaction. KIF14 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-KIF14 antibody NB100-243. For blotting immunoprecipitated KIF14, NB100-254 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for KIF14 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes bands around 190 kDa representing KIF14.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIF14

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF14 silencing experiments suggest that KIF14 plays a multi-functional role in mitosis and cytokinesis. KIF14 as also been identified as a candidate oncogene whose overexpression may lead to genomic instability and act as a reporter of poor-prognosis in breast cancer. Alternate identifiers for KIF14 include kinesin family member 14, kinesin-like protein KIF14, KIAA0042, and MGC142302.

Alternate Names

KIAA0042MGC142302, kinesin family member 14, kinesin-like protein KIF14

Entrez Gene IDs

9928 (Human)

Gene Symbol

KIF14

UniProt

NP_055690.1 (Human)

Additional KIF14 Products

Product Documents for KIF14 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KIF14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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