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KIF18A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85127

Novus Biologicals, part of Bio-Techne
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NBP1-85127

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIF18A Antibody

Western Blot: KIF18A Antibody [NBP1-85127]

Western Blot: KIF18A Antibody [NBP1-85127]

Western Blot: KIF18A Antibody [NBP1-85127] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: KIF18A Antibody [NBP1-85127]

Immunohistochemistry-Paraffin: KIF18A Antibody [NBP1-85127]

Immunohistochemistry-Paraffin: KIF18A Antibody [NBP1-85127] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: KIF18A Antibody [NBP1-85127]

Western Blot: KIF18A Antibody [NBP1-85127]

Western Blot: KIF18A Antibody [NBP1-85127] - Analysis in control (vector only transfected HEK293T lysate) and KIF18A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for KIF18A Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KIF18A

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF18 has been demonstrated to be a microtubule depolymerase essential for chromosome alignment at the spindle equator. Alternate names for KIF18A include kinesin family member 18A, kinesin-like protein KIF18A, and DKFZP434G2226.

Long Name

Kinesin-like protein KIF18A

Alternate Names

MS-KIF18A, OK/SW-cl.108

Gene Symbol

KIF18A

Additional KIF18A Products

Product Documents for KIF18A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KIF18A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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