KIF2B Antibody

Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-86002]

Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-86002] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in seminiferous ducts.

Western Blot: KIF2B Antibody [NBP1-86002] -

Western Blot: KIF2B Antibody [NBP1-86002] - TBK1 depletion alters microtubule dynamics.(a) Shcontrol A549 or H1650 cells treated with 0.1 μM nocodazole for 15 min showed complete disruption of microtubules, while TBK1-depleted shTBK1 A549 or H1650 cells retained stable microtubules as seen by staining for alpha tubulin (green) & CEP170 (red). (b) Mitotic spindles in TBK1-depleted A549 & H460 cells were more stable after nocodazole treatment as compared to control cells. Alpha tubulin (green) shows mitotic spindles. (c) shTBK1 A549 or H1650 cells when subjected to cold temperature (4 °C) & subsequent incubation at warm temperature (37 °C) had stable microtubules versus analogously treated shcontrol A549 or H1650 cells. Alpha Tubulin (green), CEP170 (red) & DNA (DAPI, Blue). (d) Similar results were observed in mitotic cells where mitotic spindles of TBK1-depleted A549 or H460 cells were more stable compared to control shRNA-treated cells. Alpha tubulin (green). (e) Stability of microtubules were drastically reduced in A549 cells overexpressing TBK1 when treated with 5 μM nocodazole & allowed to regrow for 7 min; Scale bar, 10 μm for e. (f) Low magnification images of cells in c. Scale bar, 25 μm. (g–i) TBK1 is required for the binding of CEP170 with Kif2b (g) as well as for the association of NuMA with dynein (i), as seen by immunoprecipitation–western blot analysis. (h) Western blot for pH3S10 to confirm mitotic fractions. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms10072), licensed under a CC-BY license. Not internally tested by Novus Biologicals.