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KIF2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89446

Novus Biologicals, part of Bio-Techne
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NBP1-89446
NBP1-89446-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLAVVTEINRENYWVTVEWVEKAVKKGKKIDLETILLLNPALDSAEHP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIF2B Antibody

Western Blot: KIF2B Antibody [NBP1-89446]

Western Blot: KIF2B Antibody [NBP1-89446]

Western Blot: KIF2B Antibody [NBP1-89446] - Analysis in control (vector only transfected HEK293T lysate) and KIF2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: KIF2B Antibody [NBP1-89446]

Immunocytochemistry/ Immunofluorescence: KIF2B Antibody [NBP1-89446]

Immunocytochemistry/Immunofluorescence: KIF2B Antibody [NBP1-89446] - Staining of human cell line U-2 OS shows localization to nucleoli, microtubules, cytokinetic bridge & mitotic spindle. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446]

Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446]

Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.

Applications for KIF2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KIF2B

Alternate Names

FLJ53902, kinesin family member 2B, kinesin protein, kinesin-like protein KIF2B

Entrez Gene IDs

84643 (Human)

Gene Symbol

KIF2B

UniProt

Additional KIF2B Products

Product Documents for KIF2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KIF2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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