KIFC1 Antibody (10M10J4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15248
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10M10J4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for KIFC1 Antibody (10M10J4)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIFC1 (Q9BW19). MDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KIFC1 Antibody (10M10J4)
Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248]
Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248]
Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: KIFC1 Antibody (10M10J4) [NBP3-15248]
Immunohistochemistry-Paraffin: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunohistochemistry of paraffin-embedded human appendix using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for KIFC1 Antibody (10M10J4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: KIFC1
Alternate Names
HSETMGC1202, kinesin family member C1, kinesin-like 2, Kinesin-like protein 2, kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2MGC149736, MGC149737
Gene Symbol
KIFC1
Additional KIFC1 Products
Product Documents for KIFC1 Antibody (10M10J4)
Product Specific Notices for KIFC1 Antibody (10M10J4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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