Kindlin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-523

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Reacts with residues 643-660 (VHEYIGGYIFLSTRSKDQ) of the 77 kDa human URP1 protein.

Reactivity Notes

Human. Other species not tested. Sequence is 100% conserved between almost all species (mouse, rat, canine, bovine, chimpanzee, Xenopus).

Specificity

URP-1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kindlin Antibody - BSA Free

Western Blot: Kindlin AntibodyBSA Free [NB100-523]

Western Blot: Kindlin AntibodyBSA Free [NB100-523]

Western Blot: Kindlin Antibody [NB100-523] - Detection of URP1 in 20 ug of human lung adenocarcinoma cell line NCI-H322 lysate using NB 100-523.

Applications for Kindlin Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot. The theoretical MW of this protein is ~77.4 kDa. No other application have been tested. Western blot - Use at 1:1,000 dilution. *However, the investigator should determine the optimal dilution. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kindlin

Due to a concerted effort to identify biomarkers for lung and colon carcinomas by genome-wide transcriptional profiling, the identification and cloning of one such gene as well as two additional closely related genes was achieved. Due to the strong sequence homology to the C. elegans UNC-112 a novel gene gene was named URP1, for UNC-112 related protein. Another novel related gene, URP2 and the previously discovered MIG-2 gene was also identified. Transcriptional analysis shows that only URP1 is significantly differentially regulated, being over-expressed in 70% of the colon carcinomas and 60% of the lung carcinomas tested. Quantification of URP1 expression by qRT-PCR showed up-regulation of the gene by 60-fold in lung tumors and up to nearly 6-fold in colon tumors. Northern blot analysis of URP1 indicates that normal expression is restricted to neuromuscular tissues. In contrast, the expression of URP2 appears to be confined primarily to tissues of the immune system. URP1 has the potential to be one of the most important prognostic and diagnostic markers of colon or lung cancer.

Alternate Names

C20orf42fermitin family homolog 1, chromosome 20 open reading frame 42, fermitin family homolog 1 (Drosophila), fermitin family member 1, FLJ20116, KIND1DTGCU2, kindlerin, kindlin 1, Kindlin syndrome protein, Kindlin-1, UNC112 related protein 1, UNC112A, Unc-112-related protein 1, URP1FLJ23423

Entrez Gene IDs

55612 (Human)

Gene Symbol

FERMT1

UniProt

Q9BQL6 (Human)

Additional Kindlin Products

Product Documents for Kindlin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Kindlin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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