Kinesin 5B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1059

Novus Biologicals, part of Bio-Techne
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NB100-1059
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Kinesin 5B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QPVAVRGGGGKQV corresponding to C-Terminus according to NP_004512.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Kinesin 5B Antibody

Western Blot: Kinesin 5B Antibody [NB100-1059]

Western Blot: Kinesin 5B Antibody [NB100-1059]

Western Blot: Kinesin 5B Antibody [NB100-1059] - Staining of HeLa cell lysates (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Kinesin 5B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 1.5 ug/mL
Application Notes
WB: Approx. 110 kDa band observed in Jurkat and HeLa lysates (calculated MW of 110 kDa band according to NP_004512.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kinesin 5B

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Alternate Names

Conventional kinesin heavy chain, kinesin family member 5B, kinesin-1 heavy chain, KNS1kinesin 1 (110-120kD), KNSKINH, Ubiquitous kinesin heavy chain, UKHCkinesin heavy chain

Entrez Gene IDs

3799 (Human)

Gene Symbol

KIF5B

UniProt

P33176 (Human)

Additional Kinesin 5B Products

Product Documents for Kinesin 5B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Kinesin 5B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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