Kinesin 5B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21667

Novus Biologicals, part of Bio-Techne
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NBP2-21667
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, SDS-Page, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Kinesin 5B Antibodies »

Product Specifications

Immunogen

Kinesin 5B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human Kinesin 5B protein. (Uniprot: P33176)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

The product was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Kinesin 5B Antibody

Western Blot: Kinesin 5B Antibody [NBP2-21667]

Western Blot: Kinesin 5B Antibody [NBP2-21667]

Western Blot: Kinesin 5B Antibody [NBP2-21667] - Western Blot of Kinesin 5B antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:40,000 for 1hr at RT.Block: 5% BLOTTO 1 hr at RT.Predicted/Observed size: 72 kDa, ~70 kDa for Kinesin 5B. Other band(s): degradation.
Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667]

Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667]

Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - Immunohistochemistry of Kinesin 5B antibody. Tissue: Human Brain at 40X at pH 6.
Western Blot: Kinesin 5B Antibody [NBP2-21667]

Western Blot: Kinesin 5B Antibody [NBP2-21667]

Western Blot: Kinesin 5B Antibody [NBP2-21667] - Glucocorticoid promoted microtubule destabilization and following transport impairment. The hippocampus of mice exposed to rapamycin (8 mg/kg) for 2 days before corticosterone (10 mg/kg) for 24 h was collected and lysed. alpha-tubulin was co-immunoprecipitated with an anti-kinesin-1 antibody (the left side). Expression of kinesin-1 and alpha-tubulin in total cell lysates is shown in the right side. ** indicates p < 0.01 vs. vehicle. # indicates p < 0.05 vs. corticosterone alone. n = 4. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-018-1172-y) licensed under a CC-BY license.
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Applications for Kinesin 5B Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:500
Application Notes
This product has been tested for use in SDS, western blot, and immunohistochemistry. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.

Use in ICC/IF was reported in scientific literature (PMID: 30429451).

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Kinesin 5B

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Alternate Names

Conventional kinesin heavy chain, kinesin family member 5B, kinesin-1 heavy chain, KNS1kinesin 1 (110-120kD), KNSKINH, Ubiquitous kinesin heavy chain, UKHCkinesin heavy chain

Gene Symbol

KIF5B

UniProt

P33176 (Human)

Additional Kinesin 5B Products

Product Documents for Kinesin 5B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kinesin 5B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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