Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Kinesin 5B Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 675 to 725 of human Kinesin Family Member 5B using the numbering given in entry NP_004512.1 (GeneID 3799).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kinesin 5B Antibody - BSA Free

Western Blot: Kinesin 5B Antibody [NBP2-32278]

Western Blot: Kinesin 5B Antibody [NBP2-32278]

Western Blot: Kinesin 5B Antibody [NBP2-32278] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-Kinesin 5B antibody NBP2-32278 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Kinesin 5B Antibody [NBP2-32278]

Immunoprecipitation: Kinesin 5B Antibody [NBP2-32278]

Immunoprecipitation: Kinesin 5B Antibody [NBP2-32278] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-Kinesin 5B antibody NBP2-32278 used for IP at 6 ug per reaction. For blotting immunoprecipitated Kinesin 5B was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for Kinesin 5B Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kinesin 5B

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Alternate Names

Conventional kinesin heavy chain, kinesin family member 5B, kinesin-1 heavy chain, KNS1kinesin 1 (110-120kD), KNSKINH, Ubiquitous kinesin heavy chain, UKHCkinesin heavy chain

Gene Symbol

KIF5B

UniProt

NP_004512.1 (Human)

Additional Kinesin 5B Products

Product Documents for Kinesin 5B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kinesin 5B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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