Kininogen Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89993

Novus Biologicals, part of Bio-Techne
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NBP1-89993
NBP1-89993-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Kininogen Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kininogen Antibody

Western Blot: Kininogen Antibody [NBP1-89993]

Western Blot: Kininogen Antibody [NBP1-89993]

Western Blot: Kininogen Antibody [NBP1-89993] - Analysis in control (vector only transfected HEK293T lysate) and Kininogen over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Kininogen Antibody [NBP1-89993]

Immunohistochemistry-Paraffin: Kininogen Antibody [NBP1-89993]

Immunohistochemistry-Paraffin: Kininogen Antibody [NBP1-89993] - Staining of human kidney using Kininogen antibody NBP1-89993.
Western Blot: Kininogen Antibody [NBP1-89993]

Western Blot: Kininogen Antibody [NBP1-89993]

Western Blot: Kininogen Antibody [NBP1-89993] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for Kininogen Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kininogen

Kininogen, also known as 2-thiol proteinase inhibitor, is a multi-functional protein. The high molecule weight (HMW) form of kininogen is synthesized as a precursor protein (amino acid residues 1 to 644). The mature chain (residues 19 to 644) is further processed into the heavy chain (residues 19 to 380) and the light chain (residues 390 to 644) with release of the active peptide bradykinin. The heavy chain consists of three cystatin-like domains, which inhibit cysteine proteases. The light chain consists of a His-rich domain, which is associated with clotting activity.

Alternate Names

alpha 2-thiol Proteinase Inhibitor, BDK, KNG1

Gene Symbol

KNG1

Additional Kininogen Products

Product Documents for Kininogen Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kininogen Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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