Kir2.1 Antibody (S112)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12900
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S112
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
Localization
Membrane
Specificity
Detects approx 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Kir2.1 Antibody (S112)
Western Blot: Kir2.1 Antibody (S112) [NBP2-12900]
Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900]
Immunocytochemistry/Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (NBP2-12900) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900]
Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.Applications for Kir2.1 Antibody (S112)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Simple Western
1:100
Western Blot
1:1000
Application Notes
1 ug/mL of Kir2.1 Antibody was sufficient for detection of Kir2.1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. This Kir2.1 Antibody (S112B-14) is validated for Simple Western from a verified customer review. See Simple Western Antibody Database for Simple Western validation: tested in rat primary neonatal ventricular cardiomyocytes; antibody dilution of 1:100; separated by size
Reviewed Applications
Read 1 review rated 1 using NBP2-12900 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kir2.1
Long Name
Inward Rectifier K(+) Channel Kir2.1
Alternate Names
ATFB9, HIRK1, IRK1, KCNJ2, LQT7, SQT3
Gene Symbol
KCNJ2
Additional Kir2.1 Products
Product Documents for Kir2.1 Antibody (S112)
Product Specific Notices for Kir2.1 Antibody (S112)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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