KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05290

Novus Biologicals, part of Bio-Techne
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NBP3-05290
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Functional, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 33

Format

Low Endotoxin, Azide and BSA Free

Concentration

1 mg/ml

View all KIR2DL4/CD158d Antibodies »

Product Specifications

Immunogen

NK3.3 cells and KIR2DL4-Ig fusion protein

Specificity

The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of KIR2DL4/CD158d, a 45 kDa NK cell marker. Cell surface expression and function of KIR2DL4/CD158d depends on genotype of particular individuals.

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free

KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free Flow Cytometry: KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free [NBP3-05290]

Flow Cytometry: KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free [NBP3-05290]

Surface staining pattern of human lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 ug/ml, GAM APC) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Applications for KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2-6 ug/ml
Application Notes
Functional application: Activation

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIR2DL4/CD158d

KIR2DL4 (also known as 2DL4, p49, CD158d, KIR103) is a type I transmembrane protein of the killer cell Ig-like receptor (KIR) family expressed on NK and subsets of gamma-delta-T and memory/effector alpha-beta-T cells. KIR2DL4 is a unique KIR (1 - 3); alleles are not clonally restricted but are expressed codominantly (4) in all activated NK cells and constitutively on CD56^hi NK cells. KIR members with two Ig-like domains (2D) usually express domains D1 and D2, but KIR2DL4 expresses D0 and D2. Other long-tailed (L) KIR have two cytoplasmic inhibitory signaling domains (ITIM), but KIR2DL4 has one ITIM and also exhibits characteristics of activating KIR (2). An arginine within the transmembrane sequence of KIR2DL4 interacts with the signaling molecule Fc-epsilon-RI-gamma, while in activating KIR, a transmembrane lysine interacts with DAP12 (1, 5). The KIR2DL4 gene is highly polymorphic. Seven splice variants missing one or more exons have been identified, but it is not clear whether these are expressed. Several of the nine alleles identified encode a frameshift creating a prematurely truncated protein. It is estimated that up to 25% of humans do not express KIR2DL4 capable of reaching the cell surface (1, 7, 10). Human KIR2DL4 is 65 - 83% amino acid identical to other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly49 proteins are functional orthologs. Cross-linking of KIR2DL4 induces NK cells to produce IFN-gamma (6, 7); stimulation with IL-2 upregulates cell surface expression on CD56^dim cells and allows cytotoxicity (7). Although a role in immune privilege of the fetus has been suggested due to reported recognition of fetal trophoblast HLA-G by KIR2DL4 in the maternal decidua (11), subsequent data have not supported this recognition (1, 9).

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 4

Alternate Names

CD158d

Gene Symbol

KIR2DL4

Additional KIR2DL4/CD158d Products

Product Documents for KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KIR2DL4/CD158d Antibody (33) - Low Endotoxin, Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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