Kir6.1 Antibody (S366-60)

Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324]

Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324] - Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.

Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir6.1 Antibody (D) Composite.

Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.