KiSS1R/GPR54 Antibody (D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42076

Novus Biologicals, part of Bio-Techne
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NBP3-42076
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Key Product Details

Species Reactivity

Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # D12

Concentration

0.81 mg/ml

View all KiSS1R/GPR54 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Rat KiSS1R/GPR54 (Uniprot#: Q924U1; Gly327-Leu396 (Uniprot#: Q924U1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for KiSS1R/GPR54 Antibody (D12)

KiSS1R/GPR54 Antibody (D12)

Western Blot: KiSS1R/GPR54 Antibody (D12) [NBP3-42076] -

Western Blot: KiSS1R/GPR54 Antibody (D12) [NBP3-42076] - Sample: Rat Colon lysate Primary Ab: 0.4ug/ml NBP3-42076; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for KiSS1R/GPR54 Antibody (D12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.5-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

0.81 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KiSS1R/GPR54

GPR54 is a Metastin Receptor. Metastin, also known as KiSS-1, is a metastasis suppressor gene. Mutations in GPR54 have been shown to cause autosomal recessive idiopathic hypogonadotropic hypogonadism in humans and mice, suggesting that GPR54 is a key regulator for the onset of puberty (Seminara et al. 2003). GPR54 has been reported in various human brain regions, placenta, pancreas, and spinal cord, and at lower levels in spleen, peripheral blood leukocytes, testis, lymph node, thymus, small intestine, stomach, lung, and kidney. The receptor is also abundant in tumor tissues. In rat, GPR54 is expressed in liver, intestine, and in the pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex (including frontal), and striatum. ESTs have been isolated from normal placenta and kidney cancer libraries.

Long Name

KISS1 Receptor

Alternate Names

AXOR12, GPR54, Hypogonadotropin-1, Metastin Receptor, OT7T175

Gene Symbol

KISS1R

Additional KiSS1R/GPR54 Products

Product Documents for KiSS1R/GPR54 Antibody (D12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KiSS1R/GPR54 Antibody (D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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