KIST Antibody (OTI2H4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72383

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2H4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all KIST Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UHMK1 (NP_787062) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIST Antibody (OTI2H4) - Azide and BSA Free

Western Blot: KIST Antibody (OTI2H4)Azide and BSA Free [NBP2-72383]

Western Blot: KIST Antibody (OTI2H4)Azide and BSA Free [NBP2-72383]

Western Blot: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIST.
Immunohistochemistry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383]

Immunohistochemistry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383]

Immunohistochemistry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIST mouse monoclonal antibody.
Flow Cytometry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383]

Flow Cytometry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383]

Flow Cytometry: KIST Antibody (OTI2H4) - Azide and BSA Free [NBP2-72383] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIST.
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Applications for KIST Antibody (OTI2H4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KIST

Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cellcycle progression in G1 phase. May be involved in trafficking and/or processing of RNA

Alternate Names

DKFZp434C1613, EC 2.7.11.1, FLJ23015, kinase interacting with leukemia-associated gene (stathmin), Kinase interacting with stathmin, KISKIS protein kinase, KIST, serine/threonine-protein kinase Kist, U2AF homology motif (UHM) kinase 1, U2AF homology motif kinase 1

Gene Symbol

UHMK1

Additional KIST Products

Product Documents for KIST Antibody (OTI2H4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KIST Antibody (OTI2H4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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