KLF5 Antibody (HL2269) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25544
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2269
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for KLF5 Antibody (HL2269) - Azide and BSA Free
Immunogen
Recombinant fragment of human KLF5.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KLF5 Antibody (HL2269) - Azide and BSA Free
Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -
Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -
Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Knockout Validated: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -
Knockout Validated: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for KLF5 Antibody (HL2269) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF5
Long Name
Kruppel-Like Factor 5
Alternate Names
BTEB2
Gene Symbol
KLF5
Additional KLF5 Products
Product Documents for KLF5 Antibody (HL2269) - Azide and BSA Free
Product Specific Notices for KLF5 Antibody (HL2269) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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