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KLF9 Antibody (OTI8A11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45511

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8A11

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human KLF9(NP_001197) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF9 Antibody (OTI8A11)

Western Blot: KLF9 Antibody (OTI8A11) [NBP2-45511]

Western Blot: KLF9 Antibody (OTI8A11) [NBP2-45511]

Western Blot: KLF9 Antibody (8A11) [NBP2-45511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9.
Immunohistochemistry: KLF9 Antibody (OTI8A11) [NBP2-45511]

Immunohistochemistry: KLF9 Antibody (OTI8A11) [NBP2-45511]

Immunohistochemistry: KLF9 Antibody (8A11) [NBP2-45511] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) [NBP2-45511]

Immunohistochemistry: KLF9 Antibody (OTI8A11) [NBP2-45511]

Immunohistochemistry: KLF9 Antibody (8A11) [NBP2-45511] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for KLF9 Antibody (OTI8A11)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF9

KLF9 is a gene that codes for a transcription factor that activates mRNA synthesis on a selective basis from genes that only contain tandem repeats of GC boxes, otherwise the gene will be suppressed, and it has a length of 244 amino acids, and amass of approximately 27 kDa. Studies have been conducted on diseases and disorders relating to this gene, including Kabuki syndrome, otosclerosis, leiomyoma, colorectal cancer, carcinoma, glioblastoma, cholesterol, thyroiditis, and leukemia. KLF9 has also been shown to have interactions with PGR, SP1, SIN3A, HDAC7, and ELAV1 in pathways such as the diurnally regulated gene pathway.

Alternate Names

basic transcription element binding protein 1, Basic transcription element-binding protein 1, BTEB1GC-box-binding protein 1, BTEBBTE-binding protein 1, Krueppel-like factor 9, Kruppel-like factor 9, Transcription factor BTEB1

Gene Symbol

KLF9

Additional KLF9 Products

Product Documents for KLF9 Antibody (OTI8A11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF9 Antibody (OTI8A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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