KLH Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97357
Key Product Details
Species Reactivity
All Species
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
KLH (Keyhole Limpet Hemocyanin)
Localization
Secreted protein; extracellular space
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Substrate dependent
Conjugation Chemistry: Periodate
This Peroxidase Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and KLH.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Substrate dependent
Conjugation Chemistry: Periodate
This Peroxidase Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and KLH.
Scientific Data Images for KLH Antibody [HRP]
Western Blot: KLH Antibody [HRP] [NBP1-97357] - Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 ug. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TBS overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.
Applications for KLH Antibody [HRP]
Application
Recommended Usage
ELISA
1:400000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by ELISA and is assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 is suggested for this product. Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: KLH
Alternate Names
keyhole limpet hemocyanin (KLH)
Additional KLH Products
Product Specific Notices for KLH Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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