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KLHDC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82616

Novus Biologicals, part of Bio-Techne
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NBP1-82616
NBP1-82616-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for KLHDC1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FLCGGLSADNIPLSDGWIHNVTTNCWKQLTHLPKTRPRLWHTACLGKENEIMVFGGSKDDLLALDTGHCNDLLIFQTQPYSLLRSCLDCIGKNSIMLESQISLLPPKLLQQVLKKITFWAAANHREEQRVQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLHDC1 Antibody

Western Blot: KLHDC1 Antibody [NBP1-82616]

Western Blot: KLHDC1 Antibody [NBP1-82616]

Western Blot: KLHDC1 Antibody [NBP1-82616] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: KLHDC1 Antibody [NBP1-82616]

Immunocytochemistry/ Immunofluorescence: KLHDC1 Antibody [NBP1-82616]

Immunocytochemistry/Immunofluorescence: KLHDC1 Antibody [NBP1-82616] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: KLHDC1 Antibody [NBP1-82616]

Immunohistochemistry-Paraffin: KLHDC1 Antibody [NBP1-82616]

Immunohistochemistry-Paraffin: KLHDC1 Antibody [NBP1-82616] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Applications for KLHDC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLHDC1

Alternate Names

kelch domain containing 1, kelch domain-containing protein 1, MGC126644, MGC126646, MST025

Gene Symbol

KLHDC1

Additional KLHDC1 Products

Product Documents for KLHDC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLHDC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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