KLHL2 Antibody (OTI1G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02939

Novus Biologicals, part of Bio-Techne
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NBP2-02939
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G7

Concentration

0.7 mg/ml

View all KLHL2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLHL2 Antibody (OTI1G7)

Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.
Flow Cytometry: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Flow Cytometry: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.
View 1 more images

Applications for KLHL2 Antibody (OTI1G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLHL2

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Alternate Names

ABP-KELCH, Actin-binding protein Mayven, kelch, kelch (Drosophila)-like 2 (Mayven), kelch-like 2, Mayven (Drosophila), kelch-like protein 2, MAV, MAYVEN

Entrez Gene IDs

11275 (Human)

Gene Symbol

KLHL2

Additional KLHL2 Products

Product Documents for KLHL2 Antibody (OTI1G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KLHL2 Antibody (OTI1G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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