KMT1A/SUV39H1 Antibody (44.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-12405

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 44.1

Format

BSA Free

Concentration

2.0 mg/ml

View all KMT1A/SUV39H1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein, MBP-SUV39H1.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear; associates with centromeric constitutive heterochromatin

Specificity

Monoclonal Anti-SUV39H1 Histone Methyltransferase recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KMT1A/SUV39H1 Antibody (44.1) - BSA Free

Western Blot: KMT1A/SUV39H1 Antibody (44.1)BSA Free [NB120-12405]

Western Blot: KMT1A/SUV39H1 Antibody (44.1)BSA Free [NB120-12405]

Western Blot: KMT1A/SUV39H1 Antibody (44.1) - BSA Free [NB120-12405] - HeLa nuclear extract were separated on SDS-PAGE and probed with Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in Mouse, Clone: 44.1. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat and by 1:10,000 ExtrAvidin-Peroxidase.Lanes:1. 1:500 antibody2. 1:1000 antibody3. Negative Control
Immunocytochemistry/ Immunofluorescence: KMT1A/SUV39H1 Antibody (44.1) - BSA Free [NB120-12405]

Immunocytochemistry/ Immunofluorescence: KMT1A/SUV39H1 Antibody (44.1) - BSA Free [NB120-12405]

Immunocytochemistry/Immunofluorescence: KMT1A/SUV39H1 Antibody (44.1) - BSA Free [NB120-12405] - Hela cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 ug/mL Monoclonal Anti-SUV39H1 Histone Methyltransferase Clone: 44.1.The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate.

Applications for KMT1A/SUV39H1 Antibody (44.1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

2-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KMT1A/SUV39H1

In eukaryotic cells the histone methylase KMT1A / SUV39H1 and the methyl-lys binding protein HP1 interact to repress transcription at heterochromatic sites. Lys 9 of histone H3 is methylated by KMT1A, creating a binding site for the chromo domain of HP1. The Lys methylase activity of KMT1A resides in the SET domain. KMT1A and HP1 are both involved in the repressive functions of the retinoblastoma (Rb) protein. KMT1A cooperates with Rb to repress the cyclin E promoter, and in fibrobroblasts that are disrupted for KMT1A, the activity of the cyclin E and cyclin A2 genes are specifcally elevated. Chromatin IPs (ChIPs) show that Rb is necessary to direct methylation of histone H3, and is necessary for binding of HP1 to the cyclin E promoter. The KMT1A-HP1 complex is thus not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other co-repressor proteins. Histone H3 lysine 9 can be mono, di or tri methylated. Different HMTases have recently been found responsible for each modification state. G9a is thought to be responsible for all mono and di methylation of H3 K9 in slient regions of heterochromatin. In contrast, KMT1A and H2 are thought to be responsible fro the tri methylation of H3K9 found in pericentric heterochromatin.

Long Name

Histone-lysine N-methyltransferase SUV39H1

Alternate Names

EC 2.1.1.355, H3-K9-HMTase 1, KMT1A, SUV39H, SUV39H1

Entrez Gene IDs

6839 (Human); 20937 (Mouse)

Gene Symbol

SUV39H1

Additional KMT1A/SUV39H1 Products

Product Documents for KMT1A/SUV39H1 Antibody (44.1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KMT1A/SUV39H1 Antibody (44.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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