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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-175-7E8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Immunogen sequence between amino acids 720 and 780

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT2A/MLL Antibody (BL-175-7E8)

KMT2A/MLL Antibody (BL-175-7E8)

Western Blot: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] -

Western Blot: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] -Whole cell lysate (50 ug) from HEK293T, MCF-7, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MLL1 recombinant monoclonal antibody [BL-175-7E8] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
KMT2A/MLL Antibody (BL-175-7E8)

Immunoprecipitation: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] -

Immunoprecipitation: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit antiMLL1 recombinant monoclonal antibody [BL-175-7E8used for IP at 6 ul/mg lysate. MLL1 was also immunoprecipitated by a previous lot of this antibody and another antibody against a different epitope of MLL1. For blotting immunoprecipitated MLL1, was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.

Applications for KMT2A/MLL Antibody (BL-175-7E8)

Application
Recommended Usage

Immunoprecipitation

20 µl/mg lysate

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: KMT2A/MLL

MLL1 (mixed lineage leukemia 1) has been found to participate in a multitude of chromosomal translocations that are associated with a variety of hematologic malignancies. MLL1 functions as a transcriptional regulator and plays and important role in HOX gene expression during embryonic development.

Alternate Names

ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX

Gene Symbol

KMT2A

Additional KMT2A/MLL Products

Product Documents for KMT2A/MLL Antibody (BL-175-7E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KMT2A/MLL Antibody (BL-175-7E8)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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