Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KMT2A/MLL Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1320 and 1380 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT2A/MLL Antibody

Western Blot: KMT2A/MLL Antibody [NB600-249]

Western Blot: KMT2A/MLL Antibody [NB600-249]

Western Blot: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (15 and 50 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: KMT2A/MLL Antibody [NB600-249]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-249]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-249 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-248. For blotting immunoprecipitated MLL1, NB600-249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for KMT2A/MLL Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KMT2A/MLL

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Alternate Names

ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX

Entrez Gene IDs

4297 (Human); 214162 (Mouse)

Gene Symbol

KMT2A

UniProt

Q03164 (Human)

Additional KMT2A/MLL Products

Product Documents for KMT2A/MLL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KMT2A/MLL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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