KMT2A/MLL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-256
![Immunoprecipitation: KMT2A/MLL Antibody [NB600-256]](https://resources.bio-techne.com/images/products/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-256-img0013.jpg "Immunoprecipitation: KMT2A/MLL Antibody [NB600-256]")
![Western Blot: KMT2A/MLL Antibody [NB600-256]](https://resources.bio-techne.com/images/products/KMT2A-MLL-Antibody-Western-Blot-NB600-256-img0012.jpg "Western Blot: KMT2A/MLL Antibody [NB600-256]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 2725 and 2775 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KMT2A/MLL Antibody
Western Blot: KMT2A/MLL Antibody [NB600-256]
Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Flow Cytometry: KMT2A/MLL Antibody [NB600-256]](https://resources.bio-techne.com/images/products/KMT2A-MLL-Antibody-Flow-Cytometry-NB600-256-img0010.jpg "Flow Cytometry: KMT2A/MLL Antibody [NB600-256]")
Flow Cytometry: KMT2A/MLL Antibody [NB600-256]
Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).
Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).
Applications for KMT2A/MLL Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1-5 ug/ml
Immunoprecipitation
2-5 ug/mg of lysate
Simple Western
1:10
Western Blot
1:2000-1:10000
Application Notes
KMT2A/MLL antibody validated for FLOW from a verified customer review. Simple Western reported by an internal validation: tested in MCF-7, T-47D cell lines; antibody dilution of 1:10; separated by size
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using NB600-256 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: KMT2A/MLL
Alternate Names
ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX
Entrez Gene IDs
4297 (Human)
Gene Symbol
KMT2A
UniProt
Additional KMT2A/MLL Products
Product Documents for KMT2A/MLL Antibody
Product Specific Notices for KMT2A/MLL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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