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KMT2D Antibody (1D10F6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27156

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-27156-0.025ml
NBP3-27156-0.1ml

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1D10F6

Concentration

1 mg/ml

Product Summary for KMT2D Antibody (1D10F6)

Immunogen

Purified recombinant fragment of human KMT2D (AA: 445-599) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

593 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KMT2D Antibody (1D10F6)

Western Blot: KMT2D Antibody (1D10F6) [NBP3-27156] -

Western Blot: KMT2D Antibody (1D10F6) [NBP3-27156] - Analysis using KMT2D mAb against HEK293 (1) and KMT2D (AA: 445-599)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: KMT2D Antibody (1D10F6) [NBP3-27156] -

Flow Cytometry: KMT2D Antibody (1D10F6) [NBP3-27156] - Analysis of Hela cells using KMT2D mouse mAb (green) and negative control (red).

ELISA: KMT2D Antibody (1D10F6) [NBP3-27156] -

ELISA: KMT2D Antibody (1D10F6) [NBP3-27156] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Applications for KMT2D Antibody (1D10F6)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KMT2D

Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription

Alternate Names

AAD10, ALRALL1-related protein, CAGL114, EC 2.1.1.43, histone-lysine N-methyltransferase MLL2, KMT2D, Lysine N-methyltransferase 2B, MLL4, myeloid/lymphoid or mixed-lineage leukemia 2, Myeloid/lymphoid or mixed-lineage leukemia protein 2, TNRC21, trinucleotide repeat containing 21

Gene Symbol

KMT2D

Additional KMT2D Products

Product Documents for KMT2D Antibody (1D10F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KMT2D Antibody (1D10F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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