KNTC1 Antibody (10H4)
Novus Biologicals, part of Bio-Techne | Catalog # H00009735-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 10H4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KNTC1 (NP_055523, 2100 a.a. ~ 2209 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS
Reactivity Notes
Other species not tested.
Specificity
KNTC1 - kinetochore associated 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for KNTC1 Antibody (10H4)
![Western Blot: KNTC1 Antibody (10H4) [H00009735-M01]](https://resources.bio-techne.com/images/products/KNTC1-Antibody-10H4-Western-Blot-H00009735-M01-img0007.jpg "Western Blot: KNTC1 Antibody (10H4) [H00009735-M01]")
Western Blot: KNTC1 Antibody (10H4) [H00009735-M01]
Western Blot: KNTC1 Antibody (10H4) [H00009735-M01] - KNTC1 monoclonal antibody (M01), clone 10H4 Analysis of KNTC1 expression in Hela S3 NE.![Immunocytochemistry/ Immunofluorescence: KNTC1 Antibody (10H4) [H00009735-M01]](https://resources.bio-techne.com/images/products/KNTC1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-H00009735-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: KNTC1 Antibody (10H4) [H00009735-M01]")
Immunocytochemistry/ Immunofluorescence: KNTC1 Antibody (10H4) [H00009735-M01]
Immunocytochemistry/Immunofluorescence: KNTC1 Antibody (10H4) [H00009735-M01] - Analysis of monoclonal antibody to KNTC1 on HeLa cell. Antibody concentration 50 ug/ml.
Sandwich ELISA: KNTC1 Antibody (10H4) [H00009735-M01] - Detection limit for recombinant GST tagged KNTC1 is approximately 0.03ng/ml as a capture antibody.
Applications for KNTC1 Antibody (10H4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: KNTC1
Alternate Names
FLJ36151, hRod, hsROD, KIAA0166Rough Deal homolog, centromere/kinetochore protein, kinetochore associated 1, kinetochore-associated protein 1, Rod, RODHsROD, Rough deal homolog
Entrez Gene IDs
9735 (Human)
Gene Symbol
KNTC1
OMIM
607363 (Human)
UniProt
Additional KNTC1 Products
Product Documents for KNTC1 Antibody (10H4)
Product Specific Notices for KNTC1 Antibody (10H4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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