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KNTC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83032

Novus Biologicals, part of Bio-Techne
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NBP1-83032
NBP1-83032-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KNTC1 Antibody

Immunocytochemistry/ Immunofluorescence: KNTC1 Antibody [NBP1-83032]

Immunocytochemistry/ Immunofluorescence: KNTC1 Antibody [NBP1-83032]

Immunocytochemistry/Immunofluorescence: KNTC1 Antibody [NBP1-83032] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032]

Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032]

Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human tonsil shows weak cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032]

Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032]

Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.

Applications for KNTC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KNTC1

KNTC1 encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein.

Alternate Names

FLJ36151, hRod, hsROD, KIAA0166Rough Deal homolog, centromere/kinetochore protein, kinetochore associated 1, kinetochore-associated protein 1, Rod, RODHsROD, Rough deal homolog

Gene Symbol

KNTC1

Additional KNTC1 Products

Product Documents for KNTC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KNTC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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