KPNA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1373

Novus Biologicals, part of Bio-Techne
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NB100-1373
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all KPNA3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPTANLQTKEFNF corresponding to C-Terminus according to NP_002258.2.

Localization

Cytoplasmic and Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for KPNA3 Antibody

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373]

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373]

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Staining (6ug/ml) of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.
Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373]

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373]

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
KPNA3 Antibody

Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NB100-1373] -

Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NB100-1373] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Applications for KPNA3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

6 ug/ml

Peptide ELISA

detection limit 1:128000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx. 60kDa band observed in lysates of cell lines CaCo-2, HEK293 and MCF7 (calculated MW of 57.8kDa according to NP_002258.2). Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KPNA3

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

Alternate Names

hSRP1, importin alpha 4, Importin alpha Q2, importin alpha-3, importin subunit alpha-3, importin-alpha-Q2, IPOA4, karyopherin alpha 3 (importin alpha 4), Karyopherin subunit alpha-3, qip2, SRP1, SRP1gamma, SRP1-gamma, SRP4

Entrez Gene IDs

3839 (Human); 16648 (Mouse)

Gene Symbol

KPNA3

UniProt

NP_002258. (Human)

Additional KPNA3 Products

Product Documents for KPNA3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KPNA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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