KRT222 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46822

Novus Biologicals, part of Bio-Techne
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NBP2-46822
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KRT222 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YYGCIQGGKKDKKPTTSRVGFVLPSAIINEISFTTKVPQKYENENVETVTKQAILNGSIVKESTEAHGTIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KRT222 Antibody

Western Blot: KRT222 Antibody [NBP2-46822]

Western Blot: KRT222 Antibody [NBP2-46822]

Western Blot: KRT222 Antibody [NBP2-46822] - Analysis in control (vector only transfected HEK293T lysate) and KRT222 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: KRT222 Antibody [NBP2-46822]

Immunocytochemistry/ Immunofluorescence: KRT222 Antibody [NBP2-46822]

Immunocytochemistry/Immunofluorescence: KRT222 Antibody [NBP2-46822] - Staining of human cell line A549 shows localization to vesicles.
Immunohistochemistry: KRT222 Antibody [NBP2-46822]

Immunohistochemistry: KRT222 Antibody [NBP2-46822]

Immunohistochemistry: KRT222 Antibody [NBP2-46822] - Staining of human rectum shows strong cytoplasmic positivity in endocrine cells.

Applications for KRT222 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KRT222

Alternate Names

keratin 222, truncated type I keratin KA21

Entrez Gene IDs

125113 (Human)

Gene Symbol

KRT222

UniProt

Q8N1A0 (Human)

Additional KRT222 Products

Product Documents for KRT222 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KRT222 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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