Ku70/XRCC6 Antibody (3D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00002547-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ku70/XRCC6 Antibodies »

Product Specifications

Immunogen

XRCC6 (AAH18259, 1 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDTISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

Specificity

XRCC6 - X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa) (3D12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ku70/XRCC6 Antibody (3D12)

Western Blot: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

Western Blot: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

Western Blot: Ku70/XRCC6 Antibody (3D12) [H00002547-M05] - Analysis of XRCC6 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (3D12) [H00002547-M05] - Analysis of monoclonal antibody to XRCC6 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

ELISA: Ku70/XRCC6 Antibody (3D12) [H00002547-M05]

ELISA: Ku70/XRCC6 Antibody (3D12) [H00002547-M05] - Detection limit for recombinant GST tagged XRCC6 is 1 ng/ml as a capture antibody.

Applications for Ku70/XRCC6 Antibody (3D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Entrez Gene IDs

2547 (Human)

Gene Symbol

XRCC6

UniProt

AAH18259 (Human)

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (3D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ku70/XRCC6 Antibody (3D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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