Ku70/XRCC6 Antibody (8L6B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15358

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15358-100ul
NBP3-15358-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8L6B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ku70/XRCC6 Antibodies »

Product Summary for Ku70/XRCC6 Antibody (8L6B6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-609 of human Ku70/XRCC6 (P12956). PEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ku70/XRCC6 Antibody (8L6B6)

Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Western blot analysis of extracts of various cell lines, using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Immunofluorescence analysis of U-2 OS cells using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
View 1 more images

Applications for Ku70/XRCC6 Antibody (8L6B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (8L6B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ku70/XRCC6 Antibody (8L6B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.