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Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34663MFV500

Novus Biologicals, part of Bio-Techne
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NBP2-34663MFV500

Key Product Details

Species Reactivity

Human, Chicken (Negative), Mouse (Negative), Primate, Rat (Negative)

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KU729

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Nuclear extract of human HL-60 cells

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Specificity

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Marker

Nuclear Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE] -

Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Applications for Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ku70/XRCC6

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ku70/XRCC6 Antibody (KU729) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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