Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14018
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # XRCC5/7317
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa300-500) of human Ku80/XRCC5 (exact sequence is proprietary) (Uniprot: P13010 )
Localization
Nucleus, Nucleoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13796).
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 80-86kDa
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 80-86kDa
Scientific Data Images for Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
Ku80/XRCC5 Antibody (XRCC5/7317)
Formalin-fixed, paraffin-embedded human lymph node stained with Ku80/XRCC5 antibody (XRCC5/7317). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Ku80/XRCC5 Antibody (XRCC5/7317)
Formalin-fixed, paraffin-embedded human kidney stained with Ku80/XRCC5 antibody (XRCC5/7317). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Ku80/XRCC5
As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.
Long Name
Lupus Ku Autoantigen Protein 86
Alternate Names
CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5
Gene Symbol
XRCC5
Additional Ku80/XRCC5 Products
Product Documents for Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
Product Specific Notices for Ku80/XRCC5 Antibody (XRCC5/7317) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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