Kv3.2 Antibody (S410-17)

Western Blot: Kv3.2 Antibody (S410-17) [NBP2-59328]

Western Blot: Kv3.2 Antibody (S410-17) [NBP2-59328] - Analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17. Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane.Lane 4: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain .

Immunocytochemistry/ Immunofluorescence: Kv3.2 Antibody (S410-17) [NBP2-59328]

Immunocytochemistry/Immunofluorescence: Kv3.2 Antibody (S410-17) [NBP2-59328] - Analysis using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kv3.2 Antibody (D) Composite.