Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # UJ127.11

Format

BSA Free

Concentration

1 mg/ml

View all L1CAM Antibodies »

Product Specifications

Immunogen

Homogenous suspension of 16 week human foetal brain.

Localization

Cell Membrane

Marker

Axon Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for L1CAM Antibody (UJ127.11) - BSA Free

Western Blot: L1CAM Antibody (UJ127.11)BSA Free [NB100-2682]

Western Blot: L1CAM Antibody (UJ127.11)BSA Free [NB100-2682]

Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (UJ127.11) - BSA Free [NB100-2682]

Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (UJ127.11) - BSA Free [NB100-2682]

Immunocytochemistry/Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti- NB100-2682 at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Frozen: L1CAM Antibody (UJ127.11) - BSA Free [NB100-2682]

Immunohistochemistry-Frozen: L1CAM Antibody (UJ127.11) - BSA Free [NB100-2682]

Immunohistochemistry-Frozen: L1CAM Antibody (UJ127.11) [NB100-2682] - Human pancreatic ductal adenocarcinoma (PDAC) tissue section stained with L1CAM antibody. IHC-Fr image submitted by a verified customer review.
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Applications for L1CAM Antibody (UJ127.11) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow (Cell Surface)

2.5 ug/mL

Flow Cytometry

2.5 ug/mL

Immunocytochemistry/ Immunofluorescence

2 - 5 ug/mL

Immunofluorescence

1 - 2 ug/mL

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/mL
Application Notes
Positive control(s): Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-2682 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: L1CAM

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for L1CAM Antibody (UJ127.11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for L1CAM Antibody (UJ127.11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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