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L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20137

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20137-0.025mg
NBP3-20137-0.2mg

Key Product Details

Species Reactivity

Validated:

Human

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RAB-C213

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Immunogen

This antibody was obtained by recombinant antibody (rAb) phage display recognizing L3MBTL1 protein under non-denaturing conditions.

Epitope

This antibody binds to a folded domain, amino acids 200-530.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free [NBP3-20137] -

Immunocytochemistry/Immunofluorescence: L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free [NBP3-20137] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C213 (NBP3-20137) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20137, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Flow Cytometry: L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free [NBP3-20137] -

Flow Cytometry: L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free [NBP3-20137] - Paraformaldehyde fixed Kelly cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C213 (NBP3-20137, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: L3MBTL1

L3MBTL1 encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

dJ138B7.3, FLJ41181, H-L(3)MBT, H-l(3)mbt protein, KIAA0681L3MBTLDKFZp586P1522, l(3)mbt (Drosophila)-like, L(3)mbt protein homolog, L(3)mbt-like, l(3)mbt-like (Drosophila), l(3)mbt-like 1 (Drosophila), L3MBT, lethal (3) malignant brain tumor l(3), lethal(3)malignant brain tumor-like protein

Gene Symbol

L3MBTL1

Additional L3MBTL1 Products

Product Documents for L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for L3MBTL1 Antibody (RAB-C213) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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