Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71121

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Lactate Dehydrogenase A/LDHA Antibodies »

Product Summary for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)Azide and BSA Free [NBP2-71121]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)Azide and BSA Free [NBP2-71121]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121]

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121]

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)Azide and BSA Free [NBP2-71121]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)Azide and BSA Free [NBP2-71121]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Applications for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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