Lactate Dehydrogenase A/LDHA Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48336V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lactate Dehydrogenase A/LDHA Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Localization

Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Lactate Dehydrogenase A/LDHA Antibody [DyLight 405]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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