Lactate Dehydrogenase B Antibody (14D7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59441

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 14D7

Format

BSA Free

Concentration

1 mg/ml

View all Lactate Dehydrogenase B/LDHB Antibodies »

Product Summary for Lactate Dehydrogenase B Antibody (14D7) - BSA Free

Immunogen

Recombinant human Lactate Dehydrogenase B (1-334aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Lactate Dehydrogenase B Antibody (14D7) - BSA Free

Western Blot: Lactate Dehydrogenase B Antibody (14D7)BSA Free [NBP2-59441]

Western Blot: Lactate Dehydrogenase B Antibody (14D7)BSA Free [NBP2-59441]

Western Blot: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Lane 1: Recombinant LDHB protein, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: K562 cell lysates, Lane 5: Jurkat cell lysates
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody (14D7) - BSA Free [NBP2-59441]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody (14D7) - BSA Free [NBP2-59441]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Lactate Dehydrogenase B Antibody (14D7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase B/LDHB

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Gene Symbol

LDHB

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Lactate Dehydrogenase B Antibody (14D7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase B Antibody (14D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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