Lactate Dehydrogenase B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55415
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0001.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen is a synthetic peptide directed towards the C-terminal region of Human Lactate Dehydrogenase B. Peptide Sequence MYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKD. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Lactate Dehydrogenase B Antibody
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0011.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]
Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12![Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-55415-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-55415]")
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-55415]
Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human lung adenocarcinoma cell line A549, 2.5.![Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-NBP1-55415-img0006.jpg "Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415]")
Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415]
Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human Placenta Antibody Concentration: 5 ug/ml.![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0002.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0007.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0008.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0010.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]](https://resources.bio-techne.com/images/products/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0009.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415]")
Applications for Lactate Dehydrogenase B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lactate Dehydrogenase B/LDHB
Alternate Names
Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5
Gene Symbol
LDHB
UniProt
Additional Lactate Dehydrogenase B/LDHB Products
Product Documents for Lactate Dehydrogenase B Antibody
Product Specific Notices for Lactate Dehydrogenase B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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