Lactoferrin Antibody (a-bC-lobe)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11196

Novus Biologicals, part of Bio-Techne
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NBP3-11196
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Key Product Details

Species Reactivity

Bovine

Applications

Immunoassay, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # a-bC-lobe

Concentration

0.1 mg/ml

View all Lactoferrin Antibodies »

Product Specifications

Immunogen

Monoclonal antibody a-bC-lobe, anti bovine Lactoferrin (Lf) is highly specific for bovine Lactoferrin.

Epitope

The 'WNIPMGL' sequence (467-473 of bovine lactoferrin) is the antigenic determinant or epitopic site of the anti C-lobe antibody a-bC-lobe.

Reactivity Notes

The antibody shows weak reactivity with human lactoferrin and korean goat lactoferrin, slight cross reactivity is seen with bovine transferrin, whereas no cross reactivity is seen with human transferrin and chicken ovotransferrin.

Specificity

The monoclonal antibody a-bC-lobe shows strong reactivities with both native and denatured forms of bovine lactoferrin and C-lobe.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Lactoferrin Antibody (a-bC-lobe)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Lactoferrin

Monoclonal antibody a-bC-lobe, anti bovine Lactoferrin (Lf) is highly specific for bovine Lactoferrin. This protein is a member of the transferrin family of metal-binding proteins found in milk and other secretory fluids and also in blood. It shows multifunctional properties of which the bacteriostatic and bactericidal effects are the best known. The molecule is constructed with a N-terminal half molecule (N-lobe) and a C-terminal half molecule (C-lobe), each of which is composed of two domains. The biologically important functions have been found mainly in the N-lobe. The lactoferrin determinants responsible for binding to Ca2+-dependent receptor on hepatocytes are present within the C-lobe. The monoclonal antibody a-bC-lobe shows strong reactivities with both native and denatured forms of bovine lactoferrin and C-lobe. The 'WNIPMGL' sequence (467-473 of bovine lactoferrin) is the antigenic determinant or epitopic site of the anti C-lobe antibody a-bC-lobe. The antibody shows weak reactivity with human lactoferrin and korean goat lactoferrin, slight cross reactivity is seen with bovine transferrin, whereas no cross reactivity is seen with human transferrin and chicken ovotransferrin.

Alternate Names

GIG12, HEL110, HLF2, Kaliocin-1, Lactoferricin, Lactoferroxin, Lactotransferrin, LF, LTF, Talalactoferrin

Gene Symbol

LTF

Additional Lactoferrin Products

Product Documents for Lactoferrin Antibody (a-bC-lobe)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lactoferrin Antibody (a-bC-lobe)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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