Lactoperoxidase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61572

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Bovine, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.7 mg/ml

View all Lactoperoxidase Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human Lactoperoxidase protein (within residues 300-400). [Swiss-Prot# P22079]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep (81%).

Localization

Secreted, extracellular space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactoperoxidase Antibody - BSA Free

Western Blot: Lactoperoxidase AntibodyBSA Free [NB110-61572]

Western Blot: Lactoperoxidase AntibodyBSA Free [NB110-61572]

Western Blot: Lactoperoxidase Antibody [NB110-61572] - Detection of LPO in MDA-MB231 cell lysate using NB110-61572.

Applications for Lactoperoxidase Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactoperoxidase

Lactoperoxidase (LPO) is a heme-containing glycoprotein enzyme of about 78 kD molecular size expressed highly in mammary gland, salivary gland and bronchial submucosal glands, and consequently found in large amounts in secretory fluids such as milk, tears, saliva, and lung fluids. Lactoperoxidase belongs to mammalian peroxidase family with a prosthetic heme group covalently linked to the proteins and other members of this family include myeloperoxidase (MPO), eosinophil peroxidase (EPO), and thyroid peroxidase (TPO). In the presence of H2O2, lactoperoxidase oxidizes thiocyanate (SCN-) more efficiently than other peroxidases, whereas MPO, EPO, and TPO most efficiently oxidize chloride, bromide, and iodide, respectively, to form hypo(pseudo)halides. After extracellular transport, SCN- gets oxidized either by peroxidases to form hypothiocyanite (OSCN-) or nonenzymatically by hypochlorite (OCl-). SCN exporters are the cystic fibrosis transmembrane conductance regulators, calcium-activated chloride channels, and pendrin/Slc26A4. OSCN- is tissue-innocuous but has a broad-spectrum microbicidal and microbistatic activity.

Alternate Names

EC 1.11.1, lactoperoxidase, MGC129990, MGC129991, Salivary peroxidase, SAPX, SPOEC 1.11.1.7

Gene Symbol

LPO

Additional Lactoperoxidase Products

Product Documents for Lactoperoxidase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lactoperoxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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