Lactoperoxidase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61574

Novus Biologicals, part of Bio-Techne
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NB110-61574
NB110-61574SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Goat, Sheep

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.5 mg/ml

View all Lactoperoxidase Antibodies »

Product Summary for Lactoperoxidase Antibody - BSA Free

Immunogen

Synthetic peptide made to a C-terminal portion of the human Lactoperoxidase protein (within residues 650-712). [Swiss-Prot# P22079]

Localization

Secreted, extracellular space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactoperoxidase Antibody - BSA Free

Western Blot: Lactoperoxidase AntibodyBSA Free [NB110-61574]

Western Blot: Lactoperoxidase AntibodyBSA Free [NB110-61574]

Western Blot: Lactoperoxidase Antibody [NB110-61574] - Detection of LPO in MDA-MB231 cell lysate

Applications for Lactoperoxidase Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactoperoxidase

Lactoperoxidase is an antibacterial agent in cow milk. The heme protein lactoperoxidase (LPO), also referred to as salivary peroxidase (SPO), is an oxidoreductase secreted into milk. LPO belongs to the XPO subfamily of the peroxidase family. It is expressed in mammary and salivary glands, and in the presence of H2O2, LPO acts as a catalyst for the oxidation of many phenols and aromatic amines. It is crucial for protecting the lactating mammary gland and intestinal tract of newborn infants against microorganisms. LPO binds one calcium ion per heterodimer and one heme B (iron-protoporphyrin IX) group covalently per heterodimer. The LPO gene, which spans 28 kb, is similar in gene organization and sequence to the peroxidase genes MPO and EPX, suggesting the possibility that these genes evolved from a common ancestral gene. The LPO and MPO genes are arranged in a tail-to-tail manner on chromosome 17q23.1.

Alternate Names

EC 1.11.1, lactoperoxidase, MGC129990, MGC129991, Salivary peroxidase, SAPX, SPOEC 1.11.1.7

Gene Symbol

LPO

Additional Lactoperoxidase Products

Product Documents for Lactoperoxidase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lactoperoxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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