Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Lambda Light Chain Antibodies »

Product Specifications

Immunogen

Mouse paraproteins with lambda 1 and lambda 2 light chains

Reactivity Notes

Human reactivity reported in (PMID: 31350616).

Specificity

LAMBDA LIGHT CHAIN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lambda Light Chain Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody [NB100-62139] - Mouse spleen section was stained with Goat Anti-Mouse Lambda-UNLBfollowed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP Red AP, and hematoxylin.
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the TRITC conjugate of NB100-62139. Multiple staining of peripheral blood lymphocytes.
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis of BALB/c splenocytes by multiple stainnig.
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Applications for Lambda Light Chain Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lambda Light Chain Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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