Lamin A + C Antibody (133A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97688

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 133A2

Concentration

1 mg/ml

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.

Specificity

This antibody recognizes an epitope located between residues 598-611 of lamin A and therefore it reacts exclusively with lamin A.

Marker

Nuclear Envelope Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Lamin A + C Antibody (133A2)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina
Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688] - Analysis of human colon.
Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688]

Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688] - Frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells

Applications for Lamin A + C Antibody (133A2)

Application
Recommended Usage

Flow Cytometry

1:100 - 1:200

Immunohistochemistry-Frozen

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100 - 1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry on permeabilized cells, immunohistochemistry on frozen sections and paraffin embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunocytochemistry, and immunohistochemistry with fluorescent secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

This antibody is CyTOF ready..

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS Since this antibody has to be purified from the culture medium by ion-exchange chromatography it will contain remnants of bovine serum albumin.

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Gene Symbol

LMNA

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (133A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lamin A + C Antibody (133A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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