I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

Name: Lamin A + C Antibody (mab636)
Brand: Novus Biologicals
SKU: NB100-74451

We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Lamin A + C Antibody (mab636)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74451

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # mab636

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Porcine lamin preparation.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 2209722). Mouse reactivity reported in scientific literature (PMID: 11373684).

Localization

Nuclear

Specificity

Detects lamin A/C from human, bovine and porcine samples.

Marker

Nuclear Envelope Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Lamin A + C Antibody (mab636)

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of 10ug nuclear extract from fractionated mouse tissue lysate.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (red) in HeLa cells at high magnification.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in HMVEC Cells.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in A549 Cells.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

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Applications for Lamin A + C Antibody (mab636)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Western Blot

1:100 - 1:2000

Application Notes

WB: Detects an approx. 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human); 100126859 (Porcine)

Gene Symbol

LMNA

UniProt

Q3ZD69 (Porcine)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (mab636)

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Lamin A + C Antibody (mab636)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lamin A + C Antibody (mab636)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74451

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Specificity

Marker

Clonality

Host

Isotype

Scientific Data Images for Lamin A + C Antibody (mab636)

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Applications for Lamin A + C Antibody (mab636)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Lamin A + C

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (mab636)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Lamin A + C Antibody (mab636)