I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

Name: Lamin A + C Antibody
Brand: Novus Biologicals
SKU: NB100-56649

We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Lamin A + C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56649

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bat (94%), Bovine (94%), Chinese Hamster (100%), Ferret (100%), Mink (100%), Mouse (100%), Porcine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 249-265 CRAQHEDQVEQYKKELEK of Human Lamin A/C was used immunogen.

Localization

Nucleus, Nucleus Envelope/Lamina

Specificity

Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this antibody is expected to detect multiple bands in WB.

Marker

Nuclear Envelope Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin A + C Antibody

Western Blot: Lamin A + C Antibody [NB100-56649]

Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NB100-56649]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).

Flow (Intracellular): Lamin A + C Antibody [NB100-56649]

Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).

Applications for Lamin A + C Antibody

Application

Recommended Usage

Flow (Intracellular)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500 - 1:1000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera with PBS and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamin A and lamin C are alternative splicing products of the lamin A/C gene that is responsible for autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD) (1). Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope (2).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human); 16905 (Mouse); 44161 (Rat)

Gene Symbol

LMNA

UniProt

P02545 (Mouse)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Lamin A + C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lamin A + C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56649

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Marker

Clonality

Host

Isotype

Scientific Data Images for Lamin A + C Antibody

Western Blot: Lamin A + C Antibody [NB100-56649]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NB100-56649]

Flow (Intracellular): Lamin A + C Antibody [NB100-56649]

Applications for Lamin A + C Antibody

Flow (Intracellular)

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Lamin A + C

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Lamin A + C Antibody