Lamin A + C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25153

Novus Biologicals, part of Bio-Techne
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NBP2-25153
NBP2-25153-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Recombinant full length purified Lamin A + C from E. coli

Localization

Nucleus. Nucleus envelope.

Marker

Nuclear Envelope Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin A + C Antibody

Western Blot: Lamin A + C Antibody [NBP2-25153]

Western Blot: Lamin A + C Antibody [NBP2-25153]

Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-25153]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-25153]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Applications for Lamin A + C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000

Immunohistochemistry

1:5000

Western Blot

1:5000
Application Notes
This Lamin A + C antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where two strong clean bands at 74 kDa and 65 kDa, corresponding to Lamins A and C.

Reviewed Applications

Read 1 review rated 5 using NBP2-25153 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.07% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The Lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some Laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of Lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging.

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human)

Gene Symbol

LMNA

OMIM

150330 (Human)

UniProt

P02545 (Mouse)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lamin A + C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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