Lamin B1 Antibody (4B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00004001-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lamin B1 Antibodies »

Product Specifications

Immunogen

LMNB1 (NP_005564.1, 107 a.a. ~ 186 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLAD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

LMNB1 - lamin B1 (4B10)

Marker

Nuclear Envelope Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Lamin B1 Antibody (4B10)

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.
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Applications for Lamin B1 Antibody (4B10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lamin B1

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

Alternate Names

ADLD, LMN2, LMNB1

Entrez Gene IDs

4001 (Human)

Gene Symbol

LMNB1

UniProt

NP_005564 (Human)

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (4B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lamin B1 Antibody (4B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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