Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44416

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3112

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Laminin alpha 3 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q16787, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Analysis in human stomach and lymph node tissues using NBP3-44416 antibody. Corresponding Laminin alpha 3 RNA-seq data are presented for the same tissues.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human colon shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human oral mucosa shows moderate positivity in basement membrane of squamous epithelial cells.
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Applications for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 3

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq)

Alternate Names

BM600, BM600 150kD subunit, BM600-150kDa, E170, epiligrin, Epiligrin 170 kDa subunit, epiligrin alpha 3 subunit, Epiligrin subunit alpha, kalinin 165kD subunit, Kalinin subunit alpha, kalinin-165kDa, lama3a, laminin subunit alpha-3, laminin, alpha 3, laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin), laminin-5 alpha 3 chain, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, LAMNA, LOCS, nicein 150kD subunit, Nicein subunit alpha, nicein-150kDa

Gene Symbol

LAMA3

Additional Laminin alpha 3 Products

Product Documents for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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